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SyngenisLabs Ltd
Calculators

Oligo properties calculator

Enter a DNA or RNA sequence to calculate the properties you need for design and handling. The same thermodynamic modelling runs, at greater depth, inside Syngenis One.

Your sequence

Enter a sequence

Only valid bases are counted (A, C, G, T). Spaces and numbers are ignored.

Results

Calculated properties

Length
21 nt
GC content
28.6%
Melting temperature (Tm)
44.6 °C
GC estimate (Marmur–Doty style)
Molecular weight
6437.3 g/mol
Anhydrous, single strand
Extinction coefficient
221,200
L / (mol·cm) at 260 nm, nearest neighbour
nmol per OD₂₆₀
4.5
≈ 29.1 µg per OD
Base composition: A:9 C:3 G:3 T:6

Values are estimates for handling and design. Tm depends on strand and salt concentration; Syngenis One uses full nearest neighbour thermodynamics.

Design and order in one workflow

Take your sequence into Syngenis One to model chemistry and off target risk, then order synthesis ready oligos.